sarcomere mhc  (Developmental Studies Hybridoma Bank)

Journal: eLife

Article Title: Styxl2 regulates de novo sarcomere assembly by binding to non-muscle myosin IIs and promoting their degradation

doi: 10.7554/eLife.87434

Figure Lengend Snippet:

Article Snippet: The primary antibodies used include anti-Flag (Sigma, F7425), anti-HA (Santa Cruz, sc-805), anti-α-Actinin (Sigma, A7732), anti-Prox1 (Angiobio, 11–002 P), anti-slow myosin heavy chain (DSHB, F59), anti-myosin heavy chain (DSHB, A4.1025), and anti-sarcomere myosin heavy chain (MHC) (DSHB, MF20).

Techniques: Transfection, Construct, Recombinant, Plasmid Preparation, Sequencing, Negative Control, Software

Journal: eLife

Article Title: Styxl2 regulates de novo sarcomere assembly by binding to non-muscle myosin IIs and promoting their degradation

doi: 10.7554/eLife.87434

Figure Lengend Snippet: ( A ) A schematic of mouse Styxl2 protein. DSPc (red): dual-specificity phosphatase, catalytic domain. The numbers denote the positions of various amino acids in mouse Styxl2 including Ser (S) –225. ( B ) The expression of Styxl2 protein in different mouse tissues. 100 μg of soluble mouse tissue lysates were subjected to Western blot analysis. GAPDH: glyceraldehyde-3-phosphate dehydrogenase. SM: skeletal muscles. ( C ) The expression of Styxl2 protein in C2C12 myoblasts before and after differentiation in culture. MHC: myosin heavy chain. ( D ) Zebrafish zygotes were either mock-injected or injected with a Styxl2-specific morpholino (MO). Sarcomeres in muscles at 48 hpf were revealed by transmission electron microscopy (TEM). Representative images were shown. Scale bar: 500 nm. ( E ) Sarcomeres in fast and slow muscles of zebrafish embryos at 24 hpf were revealed by immunostaining using specific antibodies as indicated. Ctrl: control (non-treated); MO: Styxl2 -morpholino-treated. The nuclei of slow and fast muscle fibers were counter-stained with an anti-Prox1 antibody and DAPI, respectively. Scale bar: 10 μm. Figure 1—source data 1. Original scans for the Western blot analysis in (anti-Styxl2 and anti-GAPDH). Figure 1—source data 2. A PDF file showing original scans of the relevant Western blot analysis in (anti-Styxl2 and anti-GAPDH) with highlighted bands and sample labels. Figure 1—source data 3. Original scans for the Western blot analysis in (anti-MHC, anti-Styxl2, and anti-Tubulin). Figure 1—source data 4. A PDF file showing original scans of the relevant Western blot analysis in (anti-MHC, anti-Styxl2, and anti-Tubulin) with highlighted bands and sample labels.

Article Snippet: Antibody , anti-sarcomere MHC (Mouse monoclonal) , DSHB , MF20 , IB(1:1000) IF(1:200).

Techniques: Expressing, Western Blot, Muscles, Injection, Transmission Assay, Electron Microscopy, Immunostaining, Staining

Journal: eLife

Article Title: Styxl2 regulates de novo sarcomere assembly by binding to non-muscle myosin IIs and promoting their degradation

doi: 10.7554/eLife.87434

Figure Lengend Snippet:

Article Snippet: Antibody , anti-sarcomere MHC (Mouse monoclonal) , DSHB , MF20 , IB(1:1000) IF(1:200).

Techniques: Transfection, Construct, Recombinant, Plasmid Preparation, Sequencing, Negative Control, Software